ＰＣＲ法によるＰＲＤＶの検出

DOI Web Site 被引用文献14件 参考文献10件

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タイトル別名
  • Detection of Penaeid Rod-shaped DNA Virus(PRDV) by PCR.
  • PCRホウ ニヨル PRDV ノ ケンシュツ

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We have developed a method for detection of penaeid rod-shaped DNA virus (PRDV, formerly RV-PJ), the causative agent of penaeid acute viremia (PAV) of kuruma shrimp, by polymerase chain reaction (PCR). Two pairs of PCR primers (P1/P2 and P3/P4) were prepared, based on the nucleotide sequence of the PRDV genome. PCR using either P1/P2 or P3/P4 specifically amplified PRDV DNA. When PCR products amplified with P1/P2 were subjected to nested PCR with P3/P4, a higher sensitivity was seen than with PCR using P1/P2 or P3/P4 alone. The nested PCR was capable of detecting PRDV from a symptomatic kuruma shrimp that had been experimentally infected with low doses of PRDV, excretions from experimentally infected kuruma shrimp, and other crustaceans in a culture pond where spontaneous PAV occurred.

収録刊行物

  • 魚病研究

    魚病研究 31 (2), 93-98, 1996

    日本魚病学会

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