Adherence and Infectivity of Green Fluorescent Protein-Labeled Pseudomonas plecoglossicida to Ayu Plecoglossus altivelis.

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  • Sukenda
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of TokyoM Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University
  • Wakabayashi Hisatsugu
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of TokyoM HW Fish Health Laboratory

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  • 緑色蛍光蛋白質(GFP)遺伝子を導入した Pseudomonas plecoglossicida のアユへの付着性と感染性

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Abstract

We report green fluorescent protein (GFP) as a useful reporter molecule to track the occurrence of Pseudomonas plecoglossicida in ayu Plecoglossue altivelis. The gfp gene was placed in a shuttle vector pME4510 and then three kinds of GFP vectors, pSKL01, pSKT03 and pSKN04 were constructed, in which lac, tac, and neophosphotransferase II (npt2) promoters drove the expression of GFP, respectively. A promoterless GFP vector was designated as pSKP02. P. plecoglossicida carrying pSKT03 gave the highest expression of GFP. In addition, pSKT03 was relatively stable under non-selective pressure and there was no significant changes in the in vitro growth and pathogenicity of the cells carrying this plasmid. The cells of fluorescent P. plecoglossicida attaching to the body surface of ayu were easily detected under a fluorescence microscope. It was revealed that they adhered predominantly to the microscopic injuries in the skin and fins.

Journal

  • Fish Pathology

    Fish Pathology 36 (3), 161-167, 2001

    The Japanese Society of Fish Pathology

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