Viable but Non-culturable State of Bacterial Cold-water Disease Pathogen Flavobacterium psychrophilum at Various Temperatures
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- Sugahara Kazuhiro
- Shiga Prefectural Fisheries Experimental Station Graduate School of Agriculture, Kinki University
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- Fujiwara-Nagata Erina
- Department of Fisheries, Kinki University
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- Fukuda Ayami
- Graduate School of Agriculture, Kinki University
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- Eguchi Mitsuru
- Department of Fisheries, Kinki University
Bibliographic Information
- Other Title
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- 種々の温度で培養した冷水病菌のVBNC状態への移行
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Description
Bacterial cold-water disease (BCWD) caused by Flavobacterium psychrophilum leads to heavy mortality of ayu Plecoglossus altivelis in Japan. Previously, a 28°C warmed water treatment was shown to be an effective treatment for BCWD, since F. psychrophilum can not grow and maintain colonies at this temperature. However, it was unclear whether the bacteria might resuscitate after the treatment was over. Therefore, we investigated colony formation, membrane potential and pathogenicity of F. psychrophilum at various temperatures (15°C, 23°C, 28°C and 33°C) in sterilized underground water. Within 2 days at 28°C, F. psychrophilum completely lost their colony-forming abilities but still maintained their membrane potentials. It seemed that these cells entered into viable but non-culturable (VBNC) state. However, experimental ayu infection revealed that VBNC F. psychrophilum cells were unable to cause BCWD, suggesting that the cells were progressing towards death at 28°C.
Journal
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- Fish Pathology
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Fish Pathology 45 (4), 158-163, 2010
The Japanese Society of Fish Pathology
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Keywords
Details 詳細情報について
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- CRID
- 1390282681439169664
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- NII Article ID
- 130000433250
- 10027671416
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- NII Book ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL BIB ID
- 10954207
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed