Stabilization by the mini-F fragment of a pBR322 derivative bearing the tryptophan operon in Escherichia coli.

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In an Escherichia coli K-12 strain (trpA trpE tna) cultured in LB broth without selective pressure, a pBR322 derivative bearing the E. coli tryptophan operon (pBR322-trp) was rapidly lost: after 27 cell-number doublings, only 7% cells retained both tryptophan prototrophy (Trp+) and ampicillin resistance (Apr), and 17% were Apr but Trp-. Insertion of the mini-F DNA from F factor into this plasmid effectively suppressed both the plasmid loss and the discoordinate loss of Trp+: the percentage of Trp- cells per cell-number doubling was decreased more than 100-fold. Partial derepression of the trp operon due to 3-indole acrylic acid further decreased the stability of the pBR322-trp but not that of the mini-F-inserted pBR322-trp.

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Details 詳細情報について

  • CRID
    1390282681440020096
  • NII Article ID
    130000026555
  • DOI
    10.1271/bbb1961.49.3619
    10.1080/00021369.1985.10867319
  • COI
    1:CAS:528:DyaL28Xks1CgsQ%3D%3D
  • ISSN
    18811280
    00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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