Metabolic pathway for 2-keto-L-gulonic acid formation in Gluconobacter melanogenus IFO 3293.
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- HOSHINO Tatsuo
- Department of Applied Microbiology, Nippon Roche Research Center
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- SUGISAWA Teruhide
- Department of Applied Microbiology, Nippon Roche Research Center
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- TAZOE Masaaki
- Department of Applied Microbiology, Nippon Roche Research Center
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- SHINJOH Masako
- Department of Applied Microbiology, Nippon Roche Research Center
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- FUJIWARA Akiko
- Department of Applied Microbiology, Nippon Roche Research Center
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Description
The L-sorbosone pathway for 2-keto-L-gulonic acid (2KGA) formation from L-sorbose in Gluconobacter melanogenus IFO 3293 was confirmed. L-Sorbose dehydrogenase, which catalyzes the conversion of L-sorbose to L-sorbosone, was found in the membrane fraction of the microorganism, and L-sorbosone dehydrogenase in the cytosol fraction. The latter enzyme requires NAD or NADP as a cofactor.<br> 2KGA was reduced to L-idonic acid by 2KGA reductase, but L-idonic acid was re-oxidized to 2KGA by membrane-bound L-idonate dehydrogenase.
Journal
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 54 (5), 1211-1218, 1990
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681440530816
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- NII Article ID
- 110006324923
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- NII Book ID
- AA00515312
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- COI
- 1:CAS:528:DyaK3cXlt12isbw%3D
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- ISSN
- 18811280
- 00021369
- http://id.crossref.org/issn/00021369
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed
