Purification and characterization of four trypsin inhibitors from seeds of okra, Abelmoschus esculentus L.

OGATA Fusahiro, IMAMURA Hiroshi, HIRAYAMA Kouichirou, MAKISUMI Satoru

doi:10.1080/00021369.1986.10867737

Purification and characterization of four trypsin inhibitors from seeds of okra, Abelmoschus esculentus L.

DOI DOI オープンアクセス

OGATA Fusahiro

Department of Chemistry, Faculty of Science, Kyushu University
IMAMURA Hiroshi

Department of Chemistry, Faculty of Science, Kyushu University Present address: New Drug Research & Development Department, Pharmaceutical Division
HIRAYAMA Kouichirou

Department of Chemistry, Faculty of Science, Kyushu University
MAKISUMI Satoru

Department of Chemistry, Faculty of Science, Kyushu University

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説明

Four trypsin inhibitors were isolated from seeds of okra (Abelmoschus esculentus L.) and named okra trypsin inhibitors (OTI) A, B, C, and D. The molecular weights of all the inhibitors were estimated to be about 20, 000 by gel filtration on Sephadex G-100 and SDS-polyacrylamide gel electrophoresis. All of these inhibitors inhibited bovine trypsin strongly with the formation of complexes at a 1:1 molar ratio and three (OTI-A, OTI-B, and OTI-C) of them also weakly inhibited bovine chymotrypsin, but not simultaneously. Subtilisin Carlsberg, pronase, papain, and porcine pepsin were not inhibited by these inhibitors. The trypsin inhibitory activity of all four inhibitors was suppressed by treatment with 1, 2-cyclohexanedione but not with 2, 4, 6-trinitrobenzenesulfonate, suggesting that each of the inhibitors contains an arginine residue at the reactive site.