Identification of the tryptophan residue located at the saccharide binding site of castor bean hemagglutinin.
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- ABSAR Nural
- Laboratory of Biochemistry, Faculty of-Agriculture, Kyushu University
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- YAMASAKI Nobuyuki
- Laboratory of Biochemistry, Faculty of-Agriculture, Kyushu University
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- FUNATSU Gunki
- Laboratory of Biochemistry, Faculty of-Agriculture, Kyushu University
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説明
The tryptophan residue present at the saccharide-binding site of castor bean hemagglutinin (CBH) was identified. A peptide containing a modified tryptophan residue was isolated from the tryptic digest of S-carboxymethylated B-chain obtained from an inactive derivative of CBH (2-Oxa-CBH), in which two tryptophan residues/mol were oxidized with N-bromosuccinimide, by gel filtration on a Sephadex G-50 followed by high performance liquid chromatography. Analytical data for the isolated peptide indicated that the tryptophan residue at position 131 on the B-chain was modified in 2-Oxa-CBH.<br> From these and earlier results, it is suggested that the tryptophan residue at 131 on each B-chain is closely associated with the saccharide-binding activity of CBH. The specific role of tryptophan residue at 131 in the saccharide-binding site of CBH is also discussed.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 50 (12), 3071-3076, 1986
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681440758912
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- NII論文ID
- 110006322825
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- NII書誌ID
- AA00515312
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- COI
- 1:CAS:528:DyaL2sXpvVarsA%3D%3D
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
