Comparisons of characteristics of mercury-tolerant bacterium Pseudomonas oleovorans G-1 and its mercury-sensitive mutant strain.

DOI DOI Open Access
  • HORITSU Hiroyuki
    Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
  • ITO Tetsuya
    Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

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  • Comparisons of Characteristics of Mercury-Tolerant Bacterium<i>Pseudomonas oleovorans</i>G-1 and Its Mercury-Sensitive Mutant Strain

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An Hg2+-sensitive mutant strain was isolated from an Hg2+-tolerant bacterium Pseudomonas oleovorans G-1 strain by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. The Hg2+-sensitive mutant strain was about 10-times as sensitive to Hg2+ as the parent strain. Moreover, the mutant strain was considerably more sensitive to Cr6+ than the parent strain, but it did not show an appreciable change in sensitivity to Cd2+ and Cu2+. The mutant strain was considerably more sensitive to antibiotics achromycin, chloramphenicol and streptomycin than the parent strain. A more rigid structure was observed in the cell envelope of the mutant strain than the parent strain under transmission electron microscope. Higher amounts of DNA but less protein and RNA were found in the mutant strain compared to the parent strain. Disc electrophoretic patterns showed some differences in protein bands between the parent and mutant strain.

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