Purification, Properties and Recognition Sequence of Site-specific Restriction Endonuclease from “<i>Acetobacter xylinus</i>”

DOI DOI
  • YOSHIOKA Hajime
    Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Shizuoka University
  • NAKAMURA Hiroshi
    Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Shizuoka University
  • SASAKI Joji
    Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Shizuoka University
  • TAHARA Yasutaka
    Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Shizuoka University
  • YAMADA Yuzo
    Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Shizuoka University

書誌事項

タイトル別名
  • Restriction endonuclease in acetic acid bacteria. Part II. Purification, properties and recognition sequence of site-specific restriction endonuclease from "Acetobacter xylinus".

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説明

A type II restriction endonuclease was purified from "Acetobacter xylinus" IFO 3288 by consecutive column chromatographies on heparin-Sepharose CL-6B, DEAE-Sepharose CL-6B, DNA-cellulose and Sephacryl S-400 super fine. The purified enzyme was homogeneous on gel disc electrophoresis and free of other endonuclease, exonuclease and phosphatase activities. The enzyme was optimally active at 37°C at pH7.5 and required 50-150mM NaCl for the enzyme reaction. The enzyme cleaved lambda and M13 mp7 RF DNAs at two and one site, respectively, but did not cleave pBR322, SV40 and φX174 RF DNAs. The recognition sequence for the enzyme was determined to be 5'-C-C-T-N-A-G-G-3', and the enzyme was found to cut between C and T in the sequence, being an isoschizomer of endonuclease from a blue-green alga, Microcoleus species UTEX LB2220 (MstII).

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詳細情報 詳細情報について

  • CRID
    1390282681441370624
  • NII論文ID
    130000027646
  • DOI
    10.1271/bbb1961.47.2871
    10.1080/00021369.1983.10866048
  • COI
    1:CAS:528:DyaL2cXhtlCktrw%3D
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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