Subcloning and nucleotide sequencing of an ARS site of Candida maltosa which also functions in Saccharomyces cerevisiae.
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- KAWAI Shinya
- Department of Agricultural Chemistry, The University of Tokyo
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- HWANG Chel Won
- Department of Agricultural Chemistry, The University of Tokyo
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- SUGIMOTO Masakazu
- Department of Agricultural Chemistry, The University of Tokyo
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- TAKAGI Masamichi
- Department of Agricultural Chemistry, The University of Tokyo
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- YANO Keiji
- Department of Agricultural Chemistry, The University of Tokyo
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説明
A DNA region exhibiting ARS activity in Candida maltosa was subcloned from an isolated 3.8kb DNA fragment and designated as the TRA region previously [M. Takagi et al., J. Bacterial., 167, 551 (1986)], which replicates autonomously not only in C. maltosa but also in Saccharomyces cerevisiae. It was found that a DNA fragment of about 200bp (designated as fragment 2) exhibited ARS activity in both yeasts, and nucleotide sequence analysis of this fragment revealed that it contained five 11 bp-sequences which are homologous to the consensus sequence of ARS sites of S. cerevisiae [J. R. Broach et al., Cold Spring Harbor Symp. Quant. Biol., 47, 1167 (1982)] (9 among 11 bp being identical in each).<br> Plasmids constructed using fragment 2 (pTRA2 and pTRA12) will be useful as cloning vectors, although they showed lower transforming frequencies and lower stabilities in C. maltosa than plasmids containing the TRA region (pTRA1 and pTRA11).
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 51 (6), 1587-1591, 1987
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681441842944
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- NII論文ID
- 130000025039
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- COI
- 1:CAS:528:DyaL2sXkslWqur0%3D
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- OpenAIRE
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- 使用不可