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Affinity chromatography of neutral proteinase from Streptomyces naraensis on a column of N-carbobenzoxy-L-phenylalanyl-aminohexyl-sepharose.
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- HIRAMATSU Akira
- Department of Agricultural Chemistry, Faculty of Agriculture, Ibaraki University
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Description
N-Carbobenzoxy-L-phenylalanyl-aminohexyl-Sepharose (Z-Phe-AH-Sepharose) was found to be an effective affinity adsorbent for neutral proteinase from Streptomyces naraensis. This enzyme was adsorbed on this affinity column at pH 7.0 or higher, and eluted with 0.3 M NaCl in starting buffer. The resulting enzyme appeared homogeneous on disc electrophoresis and showed considerably higher specific activity and recovery than by previous method.
Journal
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 46 (2), 371-374, 1982
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681442219776
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- NII Article ID
- 130000028132
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- COI
- 1:CAS:528:DyaL38XhslKks7c%3D
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- ISSN
- 18811280
- 00021369
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed
