Studies on the Formation of Uricase by Streptomyces

DOI

書誌事項

タイトル別名
  • Studies on the Formation of Uricase by <i>Streptomyces</i>
  • Part II. The Induced Formation of Uricase by the Resting Cells

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説明

A strain of Streptomyces sp. produced little of uricase in the cells when they were grown in a medium consisted of peptone, glucose and inorganic salts, even in the presence of urate. The cells, however, formed a large amount of the enzyme, when they were incubated with urate in K-phosphate buffer. The amount of uricase thus formed was maximum by the cells which were harvested at the middle logarithmic phase of the preliminary growth. The induced formation of uricase required K ions in addition to Mg ions and was accelerated by glucose and some other carbon sources. The enzyme formation was inhibited completely by chloramphenicol at_??_low concentration. An equimolar allantoin to urate decomposed by the cells was accumulated in the incubation mixture. More than 3.0 units of uricase per g of wet cells were produced under the best conditions known from the present experiments. The derepression of uricase formation in the resting cells incubated in the phosphate buffer was discussed.

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詳細情報 詳細情報について

  • CRID
    1390282681443208448
  • NII論文ID
    130003523266
  • DOI
    10.1271/bbb1961.34.1625
  • COI
    1:CAS:528:DyaE3MXjvFGgtg%3D%3D
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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