Production of L-Glutamic Acid from DL-Hydantoin-5-propionic Acid by Microoganisms Part I

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タイトル別名
  • Screening of L-Glutamic Acid-Producing Microorganisms and Some Optimal Conditions for Production of L-Glutamic Acid

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説明

1) Many strains of microorganisms capable of producing L-glutamic acid from DL-hydan-toin-5-propionic acid were found.<br> Among these, the active strains were Pseudo-monas dacunhae IAM 1199, Pseudomonas incognita IPR. AHH 38, Pseudomons sp. No. 42 ATCC 15447, Micrococcus subfavus FLUEGE IFO 3026, Aerobacter cloacae T-14, Aeobacter aerogenes T-13, Escherichia intermedia IPR. S-51, Achromobacter pestifer No. 80. ATCC 15445, Achromobocter cycloclastes ATCC 15446, and Bacillus brevis ATCC 8185, and the highest was Bacillus brevis ATCC 8185.<br> 2) It was newly found that these bacteria produced exclusively L-form of glutamic acid from both D- and L-form of hydantoin-5-pro-picnic acid.<br> 3) The optimal condions for producing L-glutamic acid by Bacillus brevis ATCC 8185 were as follows, the reaction pH was 9.0 and the temperature was 42°C. Under these adequate conditions using reaction mixture containing 1.0% of DL-hydantoin-5-propionic acid, intact cells of this strain converted 900 of the DL-acid to L-glutamic acid.<br> 4) Addition of DL-hydantoin-5-propionic acid to the preculture medium is necessary to obtain the highly active cells to produce L-glutamic acid.<br> 5) A pathway of producing L-glutamic acid from D-hydantoin-5-propionic acid was also discussed. The assumptive pathway is D-HPA→chemicalL-HPA→enzymaticL-glutamic acid.

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詳細情報 詳細情報について

  • CRID
    1390282681443388672
  • NII論文ID
    130003522436
  • DOI
    10.1271/bbb1961.30.27
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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