Studies on Respiratory Enzymes in Rice Kernel Part IX

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Other Title
  • Peroxidase Isoenzymes of Rice Embryo

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Seventeen peroxidase isoenzymes have been detected in the crude extract of rice embryos by polyacrylamide gel electrophoresis. Four isoenzymes out of 17 peroxidases were isolated and purified in a preparative scale by procedures involving ammonium sulfate fractionation, calcium phosphate gel treatment, ion exchange chromatography on DEAE- and CM-Sephadex, and gel filtration on Sephadex G-75 and G-100. The four isoenzymes were those as follows; isoenzyme 15 (peroxidase 556) as the major component of basic nature, two neutral isoenzymes 6 and 7, and isoenzyme 2 as an acidic peroxidase. All of the isolated peroxidases exhibited absorption spectra of high-spin type with minor differences from each other. The individual isoenzymes differed not only in enzymatic activity toward guaiacol as a hydrogen donor but also in molecular dimensions. It has been suggested that each isoenzyme may require specific hydrogen donor substance in the cellular metabolism.

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Details 詳細情報について

  • CRID
    1390282681443430272
  • NII Article ID
    130003523894
  • DOI
    10.1271/bbb1961.36.611
  • COI
    1:CAS:528:DyaE38XktlSksLs%3D
  • ISSN
    18811280
    00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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