Continuous Hydrolysis of Olive Oil by Lipase in Microporous Hydrophobic Hollow Fiber Bioreactor

DOI DOI 被引用文献6件 オープンアクセス
  • HOQ Mohammad Mozammel
    Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agriculture, Nagoya University
  • KOIKE Miyuki
    Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agriculture, Nagoya University
  • YAMANE Tsuneo
    Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agriculture, Nagoya University
  • SHIMIZU Shoichi
    Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agriculture, Nagoya University

書誌事項

タイトル別名
  • Bioreactor for enzymatic reaction of fat and fatty acid derivatives. Part IV. Continuous hydrolysis of olive oil by lipase in microporous hydrophobic hollow fiber bioreactor.
  • Continuous hydrolysis of olive oil by lipase in a microporous hydrophobic hollow fiber bioreactor
公開日
1985
DOI
  • 10.1271/bbb1961.49.3171
  • 10.1080/00021369.1985.10867235
公開者
公益社団法人 日本農芸化学会

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説明

Olive oil was hydrolyzed continuously at 40°C by Candida cylindracea lipase in a small hollow fiber bioreactor (total area of hollow fibers was about 0.11m2) in which the hollow fibers were made of microporous polypropylene. The lipase could be adsorbed easily onto oil-impregnated hollow fibers from its aqueous solution. The continuous feedings of olive oil inside the hollow fibers and of the buffer solution containing 18% glycerol as a stabilizer outside the hollow fibers were started after the enzyme-glycerol solution was removed from the bioreactor and the buffer-glycerol solution was added. An unvaried half life of 14 days of the adsorbed enzyme was observed when increasing amounts of the enzyme (1.0-5.0mg/ml) were put in, but its half-life was lowered to 6 days when the amount of the added enzyme was less (0.05 mg/ml). Free enzymes in the enzyme solutions with and without 18% glycerol retained their initial activities equally for at least 3 months at temperatures below 4°C. This suggests the feasibility of reuse of the enzyme-glycerol solution that was used in the preceding adsorption procedure after the solution was stored and supplemented with fresh enzyme. It was demonstrated by three successive cleanings and continuous hydrolyses that the used hollow fibers were regenerable. The productivity number was 0.81mg•(unit)-1•h-1, which was 26 times as great as that of the gel-entrapped lipase.

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