Studies on fungal tannase. III. Physicochemical properties of tannase of Aspergillus flavus

DOI 被引用文献1件

書誌事項

タイトル別名
  • Studies on Fungal Tannase
  • Part II. Physicochemical Properties of Tannase of <i>Aspergillus flavus</i>

この論文をさがす

説明

With the purified perparation of the tannase of Aspergillus fiavus, some physicochemical properties were investigated. The purified enzyme was homogeneous when examined in the ultracentrifuge and electrophoresis. The molecular weight determined by the sedimen. tation-diffusion method, was found to be 194, 000. The Archibald approach-to-equilibrium method gave a molecular weight of 192, 000. The enzyme was a glycoprotein containing 25.4% hexose and showed an isoelectric point in the vicinity of pH 4.0.<br> The nitrogen content of the enzyme was determined to be 12.9% by the micro-Kjeldahl method. The amino terminal amino acids were composed of approximately l mole each of alanine and arginine per mole of enzyme by the DNP-method. Evidence is presented to show that the enzyme undergoes dissociation into inactive subunits of equal size by the treatment with guanidine hydrochloride.

収録刊行物

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

  • CRID
    1390282681444404608
  • NII論文ID
    130003415369
  • DOI
    10.1271/bbb1961.32.1079
  • COI
    1:CAS:528:DyaF1MXovFaq
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ