Respiration in Organic Acid-Forming Molds

DOI

Bibliographic Information

Other Title
  • Part I. Purification of Cytochrome c, Coenzyme Q<sub>9</sub> and L-Lactic Dehydrogenase from Lactate-Forming <i>Rhizopus oryzae</i>

Abstract

Cytochrome c, coenzyme Q and lactic dehydrogenase (L-lactate: NAD oxidoreductase, EC 1, 1, 1, 27) in Rhizopus oryzae were studied in order to investigate the connection between the mechanism of lactate formation and terminal respiration.<br> Cytochrome c was extracted easily and in good yield by the addition of cetyltrimethyl ammonium bromide to mycelial suspensions. It was purified by calcium phosphate gel and Amberlite IRC-50 resin chromatography.<br> Coenzyme Q was extracted with ethanol, purified by chromatography on silicic acid, and, following crystallization from a mixture of ethanol and methanol, was identified as coenzyme Q9.<br> Lactic dehydrogenase was partially purified and some of its properties were investigated.

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Keywords

Details 詳細情報について

  • CRID
    1390282681444647680
  • NII Article ID
    130003522564
  • DOI
    10.1271/bbb1961.30.717
  • ISSN
    18811280
    00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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