Pentose Metabolism in Clostridium acetobutylicum
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- TOMOEDA Mikio
- Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
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- HORITSU Hiroyuki
- Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
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- SASAKI Ikuharu
- Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
書誌事項
- タイトル別名
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- Pentose Metabolism in <i>Clostridium acetobutylicum</i>
- Part I. L-Arabinose Ketol Isomerase
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説明
L-Arabinose ketol-isomerase (EC 5. 3. 1. 4) was extracted from the L-arabinose-grown cells of Clostridium acetobutylicum and partially purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography and the gel filtration on Sephadex G-200.<br> Optimum pH and temperature for the activity of the enzyme were 7.5 and 37°C, respectively. The enzyme was inhibited by EDTA, Na-pyrophosphate and KCN, and its activity was restored by the addition of divalent metal ions such as Mn2+ and Co2+. The Michaelis-Menten's constants for L-arabinose, Mn2+ and Co2+ were 1.08×10-2M, 0.83×10-4M and 0.38×10-4M, respectively. The equilibrium constant (K=ribulose/arabinose) was 3.14 (37◊C, pH 7.5). The thermodynamic quantities, ΔH, ΔG (37°C), ΔS (37°C) and E were +7354cal/mole, -710.7cal/mole, +26.0cal/(deg•mole) and +7471cal/mole, respectively.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 33 (2), 151-157, 1969
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681445207936
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- NII論文ID
- 130004718899
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- COI
- 1:CAS:528:DyaF1MXnsFalsQ%3D%3D
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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