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A novel one-step process for enzymatic incorporation of amino acids into proteins: Papain-catalyzed polymerization of L-methionine ethyl ester and its regulation by adding a protein substrate.
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- ARAI Soichi
- Department of Agricultural Chemistry, University of Tokyo
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- YAMASHITA Michiko
- Department of Agricultural Chemistry, University of Tokyo
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- FUJIMAKI Masao
- Department of Agricultural Chemistry, University of Tokyo Department of Food and Nutrition, Ochanomizu University
Bibliographic Information
- Other Title
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- A Novel One-step Process for Enzymatic Incorporation of Amino Acids into Proteins : Papain-catalyzed Polymerization of<scp>l</scp>-Methionine Ethyl Ester and Its Regulation by Adding a Protein Substrate
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Description
To enhance a methionine level of soy protein the plastein reaction was replaced by a simplified one-step process which had been newly developed as described in the preceding paper. It was found that during the process L-methionine ethyl ester (H•Met•OEt) underwent papain-catalyzed polymerization when the enzymatic incubation was carried out in the absence or inadequacy of soy protein. There was a critical point with regard to the ratio of the concen-tration of H•Met•OEt•HCl ([M]) and that of the soy protein ([S]). A sufficiently low [M]/[S] prevented H• Met•OEt from forming its polymer, permitting efficient incorporation of methio-nine into soy protein. A high possibility was suggested that in such a case H•Met•OEt acted as a nucleophile amine to be incorporated in the mode of papain-catalyzed aminolysis of protein.
Journal
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 43 (5), 1069-1074, 1979
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681445310720
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- NII Article ID
- 130003526462
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- COI
- 1:CAS:528:DyaE1MXksFCrurk%3D
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- ISSN
- 18811280
- 00021369
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed