Nucleotide Sequence of the<i>Clostridium stercorarium xynA</i>Gene Encoding Xylanase A: Identification of Catalytic and Cellulose Binding Domains
書誌事項
- タイトル別名
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- Nucleotide Sequence of the Clostridium stercorarium xynA Gene Encoding Xylanase A: Identification of Catalytic and Cellulose Binding Domains.
- Nucleotide sequence of <italic>Clostridium stercorarium</italic> xynA gene encoding xylanase A: Identification of catalytic and cellulose binding domains
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説明
The nucleotides of the xynA gene of Clostridium stercorarium were sequenced. The structural gene consists of an open reading frame of 1533 bp encoding 511 amino acids with an Mr of 56, 519. The signal peptide cleavage site was identified by comparison with the N-terminal amino acid sequence of the enzyme produced by a recombinant Escherichia coli. Xylanase A consists of a catalytic domain belonging to family G at the N-terminus and two direct repeats of about 90 amino acids with a short spacing at the C-terminus. Deletion analysis showed that the repeated sequences were responsible for binding the enzyme to Avicel and were not essential for catalytic activity. The catalytic domain of this enzyme is highly homologous to xylanase A of Clostridium acetobutylicum (identity: 69%) and xylanase B of Bacillus pumilus (identity: 64%).
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 57 (2), 273-277, 1993
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681447034240
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- NII論文ID
- 110002676200
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK3sXltFais7k%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- PubMed
- 7763496
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- 本文言語コード
- en
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- journal article
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- PubMed
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