Characterization of Molecular Structure of Starch Granules in Suspension-cultured Cells from Ipomoea cordatotriloba Denn.

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  • KITAHARA Kanefumi
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
  • IMAMURA Koki
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
  • OMAE Yuko
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
  • SUGANUMA Toshihiko
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

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  • Characterization of Molecular Structure of Starch Granules in Suspension-cultured Cells from<i>Ipomoea cordatotriloba</i>Denn.

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Abstract

  The molecular structure of starch granules formed in suspension-cultured cells of Ipomoea cordatotriloba Denn. was characterized by its chain length distribution, which was compared to those of the starches from the root and leaf of the original plant. The cultured cell starches were spherical and had a very small granule size (about 2 μm). The debranched starches roughly separated into three fractions during gel-permeation chromatography, and the fractions were defined as Fr.1, 2, and 3, respectively. The chain length distribution of the debranched cultured cell starch showed that the high molecular weight fraction (Fr.1), referred to as an amylose fraction, was much less than those of the root and leaf starches. The ratio of the two lower fractions (Fr.3/Fr.2) of the cultured cell starch, which was mainly derived from unit chains of amylopectin, was greatest among the starches, suggesting that the amylopectin from the cultured cell starch has much shorter unit chains. By X-ray diffraction analysis, it was found that both cultured cell and leaf starch granules have low crystallinity.<br>

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