A Transgenic Apple Callus Showing Reduced Polyphenol Oxidase Activity and Lower Browning Potential.

DOI Web Site Web Site PubMed 9 Citations 22 References

Bibliographic Information

Other Title
  • Transgenic Apple Callus Showing Reduced Polyphenol Oxidase Activity and Lower Browning Potential

Search this article

Description

Polyphenol oxidase (PPO) is responsible for enzymatic browning of apples. Apples lacking PPO activity might be useful not only for the food industry but also for studies of the metabolism of polyphenols and the function of PPO. Transgenic apple calli were prepared by using Agrobacterium tumefaciens carrying the kanamycin (KM) resistant gene and antisense PPO gene. Four KM-resistant callus lines were obtained from 356 leaf explants. Among these transgenic calli, three calli grew on the medium containing KM at the same rate as non-transgenic callus on the medium without KM. One callus line had an antisense PPO gene, in which the amount and activity of PPO were reduced to half the amount and activity in non-transgenic callus. The browning potential of this line, which was estimated by adding chlorogenic acid, was also half the browning potential of non-transgenic callus.

Journal

Citations (9)*help

See more

References(22)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top