Monitoring the Irradiation-induced Conformational Changes of Ovalbumin by Using Monoclonal Antibodies and Surface Plasmon Resonance.
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- MASUDA Tetsuya
- Research Institute for Food Science, Kyoto University
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- YASUMOTO Kyoden
- Department of Food and Nutrition, Sugiyama Jogakuen University
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- KITABATAKE Naofumi
- Research Institute for Food Science, Kyoto University
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Abstract
Two types of conformationally specific anti-irradiated ovalbumin monoclonal antibodies were prepared in order to study and monitor irradiation-induced structural changes in the ovalbumin molecule. Surface plasmon resonance (SPR) detection was used to investigate the kinetic parameters of the reaction between antibodies and ovalbumin which had been administered with different doses of irradiation (0, 1.5, 2.0, 5.0, 10, 20, 50, and 100 kGy). The results demonstrate that the combination of monoclonal antibodies and the SPR method can be used to characterize the irradiation-induced conformational change with an unlabelled reagent.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (4), 710-716, 2000
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681447614848
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- NII Article ID
- 110002679980
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXjtVelsb4%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 5379942
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- PubMed
- 10830481
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed