Ascorbic Acid Stimulation of Production of a Highly Branched β-1,3-Glucan by Aureobasidium pullulans K-1--Oxalic Acid, a Metabolite of Ascorbic Acid as the Stimulating Substance
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- HAMADA Nobutake
- Osaka Municipal Technical Research Institute
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- DEGUCHI Kohji
- Agriculture Faculty of Kinki University
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- OHMOTO Takashi
- Osaka Municipal Technical Research Institute
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- SAKAI Kiyofumi
- Osaka Municipal Technical Research Institute
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- OHE Tatsuhiko
- Osaka Municipal Technical Research Institute
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- YOSHIZUMI Hajime
- Agriculture Faculty of Kinki University
書誌事項
- タイトル別名
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- Ascorbic Acid Stimulation of Production of a Highly Branched .BETA.-1,3-Glucan by Aureobasidium pullulans K-1. Oxalic Acid, a Metabolite of Ascorbic Acid as the Stimulating Substance.
- Ascorbic Acid Stimulation of Production of a Highly Branched ベータ 1 3 Glucan by Aureobasidium pullulans K 1 Oxalic Acid a Metabolite of Ascorbic Acid as the Stimulating Substance
- Ascorbic Acid Stimulation of Production of a Highly Branched β-1,3-Glucan by<i>Aureobasidium pullulans</i>K-1—Oxalic Acid, a Metabolite of Ascorbic Acid as the Stimulating Substance—
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The production of a highly branched β-1,3-glucan by Aureobasidium pullulans K-1 in Czapek’s medium has been found to be stimulated by ascorbic acid. When the culture supernatant, after removal of polysaccharide from the culture filtrate by ethanol precipitation, was concentrated, then added to a new medium and this strain was cultured in the medium, the polysaccharide production was stimulated the same as when L-ascorbic acid was added to the medium. The stimulating substance was partially purified from the supernatant, and was found to be oxalic acid; 0.03% oxalic acid was the most effective concentration for the stimulation of polysaccharide production. The stimulating substance, oxalic acid, was proved to be derived from ascorbic acid added to a medium in an experiment using L-[1-14C]ascorbic acid. We suggest that oxalic acid generated from the metabolism of ascorbic acid in cells of Aureobasidium pullulans K-1 participated in the stimulation of the polysaccharide production by ascorbic acid.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (9), 1801-1806, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681448290688
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- NII論文ID
- 110002680169
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXnt1art7o%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5586582
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- PubMed
- 11055380
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可