Enzymatic Assay of Histamine by Amperometric Detection of H2O2 with a Peroxidase-based Sensor.

  • HIBI Takao
    Department of Bioscience, Fukui Prefectural University
  • SENDA Mitsugi
    Department of Bioscience, Fukui Prefectural University

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  • Enzymatic Assay of Histamine by Amperometric Detection of H<sub>2</sub>O<sub>2</sub>with a Peroxidase-based Sensor

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Abstract

  A method for an enzymatic assay of histamine by using histamine oxidase from Arthrobacter globiformis in combination with amperometric determination of H2O2 is described. Histamine could be quantified at a level as low as 10-7 M. The assay is adaptable to determine histamine in food samples including tuna fish with good sensitivity and selectivity.<br>

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