Purification of Stearidonic Acid (18:4(n-3)) and Hexadecatetraenoic Acid (16:4(n-3)) from Algal Fatty Acid with Lipase and Medium Pressure Liquid Chromatography.
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- ISHIHARA Kenji
- Marine Biochemistry Division, National Research Institute of Fisheries Science
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- MURATA Masakazu
- Marine Biochemistry Division, National Research Institute of Fisheries Science
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- KANENIWA Masaki
- Marine Biochemistry Division, National Research Institute of Fisheries Science
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- SAITO Hiroaki
- Marine Biochemistry Division, National Research Institute of Fisheries Science
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- KOMATSU Wataru
- Department of Life Science, Toita Women's College
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- SHINOHARA Kazuki
- National Food Research Institute
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抄録
Stearidonic acid (18:4(n-3)) and hexadecatetraenoic acid (16:4(n-3)) are included in some edible marine algae such as Undaria pinnatifida and Ulva pertusa with relatively high compositions (up to 40%) of total fatty acids. In order to prepare 16:4(n-3) and 18:4(n-3) enriched fatty acid concentrates, we screened for a suitable lipase which concentrates these acids by the removal of other fatty acids in the selective esterification reaction reported by Shimada et al. (Shimada et al. (1997), J. Am. Oil Chem. Soc., 74, 1465-1470). In combination with the lipase reaction and reversed-phase medium pressure liquid chromatography, we purified 18:4(n-3) and 16:4(n-3) to more than 95% purity.<br>
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 64 (11), 2454-2457, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681448327424
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- NII論文ID
- 110002679838
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXos1Srtbo%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5590729
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- PubMed
- 11193415
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可