Large scale production and crystallization of acid carboxypeptidase from submerged culture of Penicillium janthinellum, and stability of the crystalline enzyme.

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Acid carboxypeptidase of Penicillium janthinellum IFO-8070 was produced effectively in submerged culture on a medium of 4_??_5% rice bran. The enzyme production was enlarged to volume cultivation of 150-liters in a 200-liters jar fermentor, and the yield of acid carboxy-peptidase per milliliter filtrate reached to the maximum 3 days after inoculation.<br> Acid carboxypeptidase of low molecular weight (M. W.=51, 000) produced in the liquid culture broth was purified and crystallized in a large scale. Purification steps include Amberlite CG-50 treatment, ammonium sulfate precipitation, dialysis using “Diaflow, ” activated charcoal treatment, and condensation using collodion-bag, or condensation and dialysis using “Diaflow.”<br> The crystals of the acid carboxypeptidase suspended in 50mM acetate buffer (pH 3.7) were completely stable for six months at 5°C. On the other hand, at low enzyme concentration (0.01 U/ml) in 50mM acetate buffer (pH 3.7), crystallized enzyme was somewhat labile, whereas, this inactivation was completely depressed by covering enzyme solution with toluene.

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Details 詳細情報について

  • CRID
    1390282681448455552
  • NII Article ID
    130003415871
  • DOI
    10.1271/bbb1961.39.1211
  • COI
    1:CAS:528:DyaE2MXltFagsLg%3D
  • ISSN
    18811280
    00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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