Application of Two-dimensional Mapping for an Analysis of Galactosyllactoses in Yogurt.

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  • SAITO Tadao
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
  • TSUJI Tomoya
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
  • KITAZAWA Haruki
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
  • KAWAI Yasushi
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
  • ITOH Takatoshi
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

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  • Application of Two-dimensional Mapping

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  A two-dimensional mapping analysis was performed by HPLC for 4 kinds of standard galactosyllactoses (GLs, trisaccharide) which were assumed to be produced from lactose (galactopyranosylβ1→4 glucopyranose) in yogurt during the fermentation of lactic acid bacteria. After the pyridylamination of GLs, they were analyzed by HPLC in the reverse-phase (RP) and anion-exchange (AE) modes. The retention times of each peak obtained were converted to glucose units (GU) in RP mode for the pyridylaminated isomaltooligosaccharides (G1-3) and to relative retention time (RRT) in AE mode against pyridylaminated-isomaltotriose, and then the address data [GU, RRT] were plotted on a graph. This two-dimensional mapping method was found useful for a rapid qualitative evaluation of the chemical structure of trisaccharides formed in yogurt.<br>

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