Factors Improving<scp>l</scp>-Threonine Production by a Three<scp>l</scp>-Threonine Biosynthetic Genes-amplified Recombinant Strain of<i>Brevibacterium lactofermentum</i>

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  • Factors Improving L-Threonine Production by a Three L-Threonine Biosynthetic Genes-amplified Recombinant Strain of Brevibacterium lactofermentum.

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When a Brevibacterium lactofermentum L-threonine producer that accumulated L-lysine as well, was transformed with a recombinant plasmid carrying the indigenous hom, thrB, and thrC genes, delayed growth and plasmid instability were observed. Addition of organic nutrients, vitamins (thiamine : HCl and d-biotin), and NaCl under the optimal culture conditions solved these problems and further increased threonine production in a small jar fermentor.

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