Inhibition of Plant Transformation by Phytolaccoside B from Phytolacca americana Callus.

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  • KANZAKI Hiroshi
    Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University
  • KAGEMORI Toshihiko
    Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University
  • YAMACHIKA Yoko
    Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University
  • NITODA Teruhiko
    Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University
  • KAWAZU Kazuyoshi
    Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University

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  • Inhibition of Plant Transformation by Phytolaccoside B from<i>Phytolacca americana</i>Callus

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Abstract

  The newly established GUS expression bioassay on the callus extracts of 22 species of plants revealed that the methanol extract of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. This compound is promising for use as a biochemical probe for studies on the plant transformation mechanism.<br>

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