Purification, Characterization, and Overexpression of Psychrophilic and Thermolabile Malate Dehydrogenase of a Novel Antarctic Psychrotolerant,<i>Flavobacterium frigidimaris</i>KUC-1
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- OIKAWA Tadao
- Department of Biotechnology, Faculty of Engineering, Kansai University Kansai University High Technology Research Center
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- YAMAMOTO Noriko
- Department of Biotechnology, Faculty of Engineering, Kansai University
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- SHIMOKE Koji
- Kansai University High Technology Research Center
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- UESATO Shinichi
- Department of Biotechnology, Faculty of Engineering, Kansai University Kansai University High Technology Research Center
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- IKEUCHI Toshihiko
- Kansai University High Technology Research Center
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- FUJIOKA Toru
- Department of Biotechnology, Faculty of Engineering, Kansai University
書誌事項
- タイトル別名
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- Purification, Characterization, and Overexpression of Psychrophilic and Thermolabile Malate Dehydrogenase of a Novel Antarctic Psychrotolerant, Flavobacterium frigidimaris KUC-1
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We purified the psychrophilic and thermolabile malate dehydrogenase to homogeneity from a novel psychrotolerant, Flavobacterium frigidimaris KUC-1, isolated from Antarctic seawater. The enzyme was a homotetramer with a molecular weight of about 123 k and that of the subunit was about 32 k. The enzyme required NAD(P)+ as a coenzyme and catalyzed the oxidation of L-malate and the reduction of oxalacetate specifically. The reaction proceeded through an ordered bi–bi mechanism. The enzyme was highly susceptible to heat treatment, and the half-life time at 40 °C was estimated to be 3.0 min. The kcat⁄Km (μM−1·s−1) values for L-malate and NAD+ at 30 °C were 289 and 2,790, respectively. The enzyme showed pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. The enzyme contained 311 amino acid residues and much lower numbers of proline and arginine residues than other malate dehydrogenases.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 69 (11), 2146-2154, 2005
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681449459328
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- NII論文ID
- 130000030489
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7729098
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- KAKEN
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- 使用不可