The Effect of Pyruvate Decarboxylase Gene Knockout in<i>Saccharomyces cerevisiae</i>on<scp>L</scp>-Lactic Acid Production
-
- ISHIDA Nobuhiro
- Biotechnology Laboratory, Toyota Central R&D Labs Inc.
-
- SAITOH Satoshi
- Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co.
-
- ONISHI Toru
- Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co.
-
- TOKUHIRO Kenro
- Biotechnology Laboratory, Toyota Central R&D Labs Inc.
-
- NAGAMORI Eiji
- Biotechnology Laboratory, Toyota Central R&D Labs Inc.
-
- KITAMOTO Katsuhiko
- Department of Biotechnology, The University of Tokyo
-
- TAKAHASHI Haruo
- Biotechnology Laboratory, Toyota Central R&D Labs Inc.
書誌事項
- タイトル別名
-
- The Effect of Pyruvate Decarboxylase Gene Knockout in Saccharomyces cerevisiae on L-Lactic Acid Production
- Effect of Pyruvate Decarboxylase Gene Knockout in Saccharomyces cerevisiae on L Lactic Acid Production
この論文をさがす
抄録
A plant- and crop-based renewable plastic, poly-lactic acid (PLA), is receiving attention as a new material for a sustainable society in place of petroleum-based plastics. We constructed a metabolically engineered Saccharomyces cerevisiae that has both pyruvate decarboxylase genes (PDC1 and PDC5) disrupted in the genetic background to express two copies of the bovine L-lactate dehydrogenase (LDH) gene. With this recombinant, the yield of lactate was 82.3 g/liter, up to 81.5% of the glucose being transformed into lactic acid on neutralizing cultivation, although pdc1 pdc5 double disruption led to ineffective decreases in cell growth and fermentation speed. This strain showed lactate productivity improvement as much as 1.5 times higher than the previous strain. This production yield is the highest value for a lactic acid-producing yeast yet reported.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 70 (5), 1148-1153, 2006
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681449510656
-
- NII論文ID
- 10018531187
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 7926127
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可