Purification and Characterization of an Oligo-isomaltosaccharide Synthase from a Streptococcus sobrinus Glucosyltransferase-I Deficient Mutant.
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- SHINOZAKI-KUWAHARA Noriko
- Departments of Microbiology, Nihon University school of Dentistry at Matsudo
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- HAYAKAWA Mitsuo
- Departments of Biochemistry, Nihon University school of Dentistry at Matsudo
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- SHIROZA Teruaki
- Departments of Biochemistry, Nihon University school of Dentistry at Matsudo
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- ABIKO Yoshimitsu
- Departments of Biochemistry, Nihon University school of Dentistry at Matsudo
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- FUKUSHIMA Kazuo
- Departments of Microbiology, Nihon University school of Dentistry at Matsudo
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One glucosyltransferase (GTF)-I deficient mutant of Streptococcus sobrinus strain B13N was isolated through chemical mutagenesis with ethyl methanesulfonate, and characterized. This mutant, designated as B13N-Id, readily allowed us to purify a homogeneous oligo-isomaltosaccharide synthase (GTF-S) from its culture fluid. The purified GTF-S was only recognized with rabbit polyclonal antibody against recombinant GTF-S from an Ecsherichia coli MD124 clone expressing the B13N gtfS gene, and showed the almost same enzymatic properties as the recombinant enzyme. A double reciprocal plot of the B13N GTF-S for sucrose was biphasic, and the affinity for this substrate was high compared to that of GTF-S enzymes from other strains.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 65 (6), 1290-1295, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681449517824
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- NII論文ID
- 110002692023
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3MXkvFWiu7Y%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5828098
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- PubMed
- 11471726
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可