Isotope Ratio by HRGC-MS of Citrus junos Tanaka (Yuzu) Essential Oils: m/z 137/136 of Terpene Hydrocarbons.

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  • SAWAMURA Masayoshi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University
  • ITO Toshiki
    Department of Bioresources Science, Faculty of Agriculture, Kochi University
  • UNE Akitoshi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University
  • UKEDA Hiroyuki
    Department of Bioresources Science, Faculty of Agriculture, Kochi University
  • YAMASAKI Yuzo
    Kochi Prefectural Industrial Technology Center

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The isotope ratios of monoterpene hydrocarbons in Citrus junos Tanaka (yuzu) essential oils from different origins were determined by ordinary high-resolution gas chromatography-mass spectrometry (HRGC-MS). Both intensities of the molecular mass peaks (m/z 136) and of the isotope peaks (m/z 137) of monoterpene hydrocarbons were measured by single-ion monitoring with an MS analysis. The isotope ratios (m/z 137/136) of the ten monoterpene hydrocarbons commonly contained in citrus essential oils, α-pinene, β-pinene, sabinene, myrcene, α-phellandrene, α-terpinene, limonene, γ-terpinene, β-phellandrene and terpinolene, were determined in yuzu samples of the highest commercial quality from 42 different production districts. Statistical treatment of these data by the t-test and sign test revealed significant differences of the isotope effects in each yuzu sample. It is suggested that this technique will be applicable for evaluating the quality, genuineness and origin of citrus fruits and their products. The isotope fingerprints were also demonstrated in several citrus fruits other than the yuzu samples.

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