Contribution of Cyanide-Insensitive Respiratory Pathway, Catalyzed by the Alternative Oxidase, to Citric Acid Production in<i>Aspergillus niger</i>
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- KIRIMURA Kohtaro
- Department of Applied Chemistry, School of Science and Engineering, Waseda University
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- YODA Masashi
- Department of Applied Chemistry, School of Science and Engineering, Waseda University
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- SHIMIZU Hideki
- Department of Applied Chemistry, School of Science and Engineering, Waseda University
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- SUGANO Shuji
- Department of Applied Chemistry, School of Science and Engineering, Waseda University
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- MIZUNO Makoto
- Department of Applied Chemistry, School of Science and Engineering, Waseda University
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- KINO Kuniki
- Department of Applied Chemistry, School of Science and Engineering, Waseda University
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- USAMI Shoji
- Department of Applied Chemistry, School of Science and Engineering, Waseda University
書誌事項
- タイトル別名
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- Contribution of Cyanide-Insensitive Respiratory Pathway, Catalyzed by the Alternative Oxidase, to Citric Acid Production in Aspergillus niger.
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In Aspergillus niger, a cyanide (CN)- and antimycin A-insensitive and salicylhydroxamic acid (SHAM)-sensitive respiratory pathway exists besides the cytochrome pathway and is catalyzed by the alternative oxidase (AOX). In this study, A. niger WU-2223L, a citric acid-producing strain, was cultivated in a medium containing 120 g/l of glucose, which is the concentration usually needed for citric acid production, and the effects of 2% (v/v) methanol, an inducer of citric acid, 2 μM antimycin A, and 1 mM SHAM on AOX activities and citric acid production were investigated. The AOX activity, measured as duroquinol oxidase, was localized in the purified mitochondria regardless of the presence of any additives. When WU-2223L was cultivated with antimycin A or methanol, both citric acid production and citric acid productivity, shown as the ratio of production per mycelial dry weight, increased with the increase of both the activity of AOX and the rate of CN-insensitive and SHAM-sensitive respiration. On the other hand, when WU-2223L was cultivated with SHAM, an inhibitor of AOX, the CN-insensitive and SHAM-sensitive respiration was not detected and the citric acid production and the productivity drastically decreased, although mycelial growth was not affected. These results clearly indicated that the CN-insensitive and SHAM-sensitive respiration catalyzed by AOX, localized in the mitochondria, contributed to citric acid production by A. niger.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (10), 2034-2039, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681449608576
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- NII論文ID
- 110002679773
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXnvVOhtrg%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5557537
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- PubMed
- 11129572
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可