Chemical Structure and Molecular Weights of .ALPHA.-(1.RAR.3)-D-Glucan from Lentinus edodes.
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- ZHANG Pingyi
- Department of Chemistry, Wuhan University
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- ZHANG Lina
- Department of Chemistry, Wuhan University
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- CHENG Shuyao
- Department of Chemistry, Wuhan University
Bibliographic Information
- Other Title
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- Chemical Structure and Molecular Weights of α-(1→3)-D-Glucan from Lentinus edodes
- Chemical Structure and Molecular Weights of アルファ 1 3 D Glucan from Lentinus edodes
- Chemical Structure and Molecular Weights of α-(1→3)-<scp>D</scp>-Glucan from<i>Lentinus edodes</i>
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Abstract
α-Glucan (L-FV-II) and β-glucan (L-FV-I) were isolated from fruiting bodies of Lentinus edodes by extraction with 5% NaOH/0.05% NaBH4, then precipitation with 1 M acetic acid. The analysis results of Smith degradation, high-performance liquid chromatography (HPLC), infrared spectra (IR), 13C NMR spectra, and optical rotation indicated that L-FV-II is α-(1→3)-D-glucan with slight (1→6) branch linkages, and L-FV-I is β-(1→3)-D-glucan containing 10% protein, and with branches. The weight-average molecular weight, Mw, number-average molecular weight, Mn, radii of gyration, <s2>1/2, and second virial coefficients, A2, of L-FV-II in aqueous 0.5 M urea/0.5 M NaOH (obtained from diluting its solution in 1.0 M urea/1.0 M NaOH) and 0.25 M LiCl in dimethylsulfoxide (DMSO/0.25 M LiCl) were measured by light scattering, membrane osmometry, and size exclusion chromatography (SEC). The values of Mw and Mn for L-FV-II in 0.5 M urea/0.5 M NaOH are 24.1×104 and 10.9×104, respectively, similar to those in DMSO/0.25 M LiCl. It was proved that strong intermolecular hydrogen bonds exist in L-FV-II, resulting in its water-insolubility, but there are not any aggregates or multiple-helix structure for L-FV-II in DMSO/0.25 M LiCl. The chain of α-(1→3)-D-glucan is more extended in DMSO/0.25 M LiCl than that in the aqueous solution.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 63 (7), 1197-1202, 1999
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681449730048
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- NII Article ID
- 110002679646
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1MXlsFCntbY%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- NDL BIB ID
- 4820261
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed