Expression and Characterization of Sucrose Synthase from Mung Bean Seedlings in<i>Escherichia coli</i>
書誌事項
- タイトル別名
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- Expression and Characterization of Sucrose Synthase from Mung Bean Seedlings in Escherichia coli.
- Expression and Characterization of Sucr
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説明
The cDNA fragment coding for mung bean (Vigna radiata Wilczek) sucrose synthase was introduced into the expression vector pET-20b resulting in the construction of plasmid pEB-01. After transformation of Escherichia coli strain BL21(DE3) cells by pEB-01 and induction with isopropyl thio-β-galactoside, high level expression of the recombinant enzyme was obtained. The enzyme had a tetrameric form that conserved the activity of sucrose synthase. Although the Km and Vmax of the recombinant enzyme acting on either UDP-glucose or fructose were very close to those of the native enzyme isolated from mung bean seedlings, the Km for sucrose was higher by a factor of 10 for the recombinant enzyme. This suggests that the recombinant sucrose synthase has a tendency to synthesize sucrose, although the native enzyme catalyzes a freely reversible reaction.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (9), 1500-1503, 1997
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681449792896
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- NII論文ID
- 110002678745
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4307666
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- PubMed
- 9339551
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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