Society for Bioscience, Biotechnology, and Agrochemistry as Induced in Response to Wounding or to TMV-Infection, and Characterization of Their Promoters

DOI 機関リポジトリ Web Site Web Site PubMed 被引用文献3件 参考文献29件 オープンアクセス
  • HAYASHI Takeshi
    <i>Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>
  • KOBAYASHI Daiki
    <i>Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>
  • KARIU Tohru
    <i>Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>
  • TAHARA Maino
    <i>Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>
  • HADA Kazumasa
    <i>Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>
  • KOUZUMA Yoshiaki
    <i>Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>
  • KIMURA Makoto
    <i>Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>

書誌事項

タイトル別名
  • Genomic Cloning of Ribonucleases in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-Infection, and Characterization of Their Promoters
  • Genomic Cloning of Ribonucleases in<i>Nicotiana glutinosa</i>Leaves, as Induced in Response to Wounding or to TMV-Infection, and Characterization of Their Promoters

この論文をさがす

説明

We previously cloned two distinct cDNA clones, NGR1 and NGR3, encoding S-like ribonucleases (RNases) induced by wounding and tobacco mosaic virus (TMV) infection, respectively, in Nicotiana glutinosa leaves. To gain insight into the regulatory mechanism of the RNase genes, we analyzed nucleotide sequences of the genes ngr1 (4.1 kbp) and ngr3 (5.3 kbp), containing their structural genes as well as 5′-flanking regions. The ngr1 gene is organized in three exons with two intervening introns, and ngr3 has four exons interrupted by three introns. Primer extension analyses localized single transcription initiation sites at −32 and −99 upstream of the translation initiation codons ATG in the genes ngr1 and ngr3, respectively. The β-glucuronidase (GUS) reporter gene analysis with serial 5′-deletion mutants as well as a gel shift assay defined the wound-responsive region at residues −509 to −288 in gene ngr1 and a TMV-responsive region at the residues −401 to −174 in ngr3, respectively. Sequence search using PLACE and PlantCARE data bases showed that a wound-responsive element: the WUN-motif, occurs within the wound-responsive region in ngr1, while ngr3 contains several potential cis-regulating elements, such as the elicitor responsiveness element: the W-box, a TMV responsive element: GT1, and the WUN-motif at positions between −401 and −174. These findings suggested that some of these cis-elements may be involved in inducible expressions of ngr1 and ngr3. Furthermore, the gel shift assay suggested that the dissociation of protein factor(s) upon TMV-infection from the regulatory region may cause an inducible expression of ngr3.<br>

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (29)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ