Identification, Cloning, and Sequencing of the Genes Involved in the Conversion of D,L-2-Amino-.DELTA.2-Thiazoline-4-Carboxylic Acid to L-Cysteine in Pseudomonas sp. Strain ON-4a.

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  • OHMACHI Tetsuo
    <i>Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University</i>
  • NISHINO Mizuka
    <i>Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University</i>
  • KAWATA Maki
    <i>Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University</i>
  • EDO Namiko
    <i>Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University</i>
  • FUNAKI Hiroko
    <i>Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University</i> Present address: <i>Research and Development Division, Nippon Gene Co., Ltd.</i>
  • NARITA Megumi
    <i>Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University</i>
  • MORI Kazuyuki
    <i>Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University</i> Present address: <i>Department of Urology, Hirosaki University School of Medicine</i>
  • TAMURA Yoshiharu
    <i>Research Center, Nippon Rikagakuyakuhin Co. Ltd.</i>
  • ASADA Yoshihiro
    <i>Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University</i>

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Other Title
  • Identification, Cloning, and Sequencing of the Genes Involved in the Conversion of D,L-2-Amino-?2-Thiazoline-4-Carboxylic Acid to L-Cysteine in Pseudomonas sp. Strain ON-4a
  • Identification, Cloning, and Sequencing of the Genes Involved in the Conversion of<scp>D</scp>,<scp>L</scp>-2-Amino-<i>Δ</i><sup>2</sup>-Thiazoline-4-Carboxylic Acid to …

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The newly isolated strain Pseudomonas sp. ON-4a converts D,L-2-amino-Δ2-thiazoline-4-carboxylic acid to L-cysteine via N-carbamoyl-L-cysteine. A genomic DNA fragment from this strain containing the gene(s) encoding enzymes that convert D,L-2-amino-Δ2-thiazoline-4-carboxylic acid into L-cysteine was cloned in Escherichia coli. Transformants expressing cysteine-forming activity were selected by growth of an E. coli mutant defective in the cysB gene. A positive clone, denoted CM1, carrying the plasmid pCM1 with an insert DNA of approximately 3.4 kb was obtained, and the nucleotide sequence of a complementing region was analyzed. Analysis of the sequence found two open reading frames, ORF1 and ORF2, which encoded proteins of 183 and 435 amino acid residues, respectively. E. coli DH5α harboring pTrCM1, which was constructed by inserting the subcloned sequence into an expression vector, expressed two proteins of 25 kDa and 45 kDa. From the analyses of crude extracts of E. coli DH5α carrying deletion derivatives of pTrCM1 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by enzymatic activity, it was found that the 25-kDa protein encoded by ORF1 was the enzyme L-2-amino-Δ2-thiazoline-4- carboxylic acid hydrolase, which catalyzes the conversion of L-2-amino-Δ2-thiazoline-4-carboxylic acid to N-carbamoyl-L-cysteine, and that the 45-kDa protein encoded by ORF2 was the enzyme N-carbamoyl-L-cysteine amidohydrolase, which catalyzes the conversion of N-carbamoyl-L-cysteine to L-cysteine.<br>

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