Time-dependent Structure and Activity Changes of α-Chymotrypsin in Water/Alcohol Mixed Solvents
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- SATO Makiko
- Institute of Materials Science, University of Tsukuba
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- SASAKI Toshiya
- Institute of Materials Science, University of Tsukuba
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- KOBAYASHI Masami
- Institute of Materials Science, University of Tsukuba
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- KISE Hideo
- Institute of Materials Science, University of Tsukuba
書誌事項
- タイトル別名
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- Time-dependent Structure and Activity Changes of .ALPHA.-Chymotrypsin in Water/Alcohol Mixed Solvents.
- Time dependent Structure and Activity Changes of アルファ Chymotrypsin in Water Alcohol Mixed Solvents
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Secondary structure of α-chymotrypsin in water/ethanol was investigated by circular dichroic (CD) spectroscopy. The changes in catalytic activity were discussed in terms of structural changes of the enzyme. α-Chymotrypsin formed β-sheet structure in water/ethanol (50/50 by volume), but it was substantially less active as compared to that in water. At water/ethanol 10/90, α-chymotrypsin took on a native-like structure, which gradually changed to β conformation with concomitant loss of activity. Change of solvent composition from water/ethanol 50/50 to 90/10 or 10/90 by dilution with water or ethanol, respectively, led to partial recovery of native or native-like structure and activity. In water/methanol, α-chymotrypsin tended to form stable β-sheet structure at water/methanol ratios lower than 50/50, but the catalytic activity decreased with time. Change to α-helix structure with substantial loss in catalytic activity was observed when α-chymotrypsin was dissolved in water/2,2,2-trifluoroethanol with water contents lower than 50%. In water/2,2,2-trifluoroethanol 90/10, α-chymotrypsin initially had the CD spectrum of native structure, but it changed with time to that characteristic of β-sheet structure.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (12), 2552-2558, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681450340480
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- NII論文ID
- 110002679859
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3MXmtVOqtw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5627295
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- PubMed
- 11210116
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可