Identification of a Collagen Production-promoting Factor from an Extract of Royal Jelly and Its Possible Mechanism

DOI Web Site Web Site PubMed 13 Citations 54 References
  • KOYA-MIYATA Satomi
    Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.
  • OKAMOTO Iwao
    Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.
  • USHIO Shimpei
    Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.
  • IWAKI Kanso
    Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.
  • IKEDA Masao
    Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.
  • KURIMOTO Masashi
    Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc.

Search this article

Abstract

We have previously shown that royal jelly (RJ) promoted collagen production by skin fibroblasts in the presence of ascorbic acid-2-O-alpha-glucoside (AA-2G). In this study, we purified the honeybee RJ-derived collagen production-promoting factor (HBRJ-CPF) from an alkali-solubilized fraction of RJ by C18 reverse-phase column chromatography. The elution profile by the C18 column chromatography and the molecular mass of the purified HBRJ-CPF material coincided with those of 10-hydroxy-2-decenoic acid (10H2DA). We then examined the collagen production-promoting activities of several commercially available fatty acids contained in RJ. We found that 10H2DA and 10-hydroxydecanoic acid increased the collagen production in a dose-dependent manner. Furthermore, 10H2DA induced the fibroblast cell line, NHDF, to produce transforming growth factor-β1 (TGF-β1) which is an important factor for collagen production. As expected, the collagen production-promoting activity of 10H2DA was neutralized by the anti-TGF-β1 antibody. These result suggest that HBRJ-CPF identified as 10H2DA promoted the collagen production of AA-2G-treated fibroblasts by inducing TGF-β1 production.

Journal

Citations (13)*help

See more

References(54)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top