Production of New Restriction Endonuclease VpaK11BI Recognizing Sequence 5'-GGWCC-3' in a Haemolysin-less Mutant of Vibrio parahaemolyticus.

UENO Harumi, KITA Akihiko, MIYAHARA Michiko, ISHIWATA Naoko, MISE Katsutoshi, KATO Ikunoshin, ISHINO Yoshizumi

doi:10.1271/bbb.61.2129

Bibliographic Information

Other Title

Production of New Restriction Endonuclease<i>Vpa</i>K111BI Recognizing Sequence 5′-GGWCC-3′ in a Haemolysin-less Mutant of<i>Vibrio parahaemolyticus</i>

Description

High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5'-GGWCC-3' and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.

Journal

Bioscience, Biotechnology, and Biochemistry

Bioscience, Biotechnology, and Biochemistry 61 (12), 2129-2130, 1997

Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

Details 詳細情報について

CRID: 1390282681450456064

NII Article ID: 110002678897

NII Book ID: AA10824164

DOI: 10.1271/bbb.61.2129

ISSN: 13476947; 09168451

PubMed: 27396891

Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.2129

Text Lang: en

Data Source

JaLC
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CiNii Articles
OpenAIRE

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