Biological Activity of Brain-derived Neurotrophic Factor with Mismatched Disulfide Linkages Produced by<i>Escherichia coli</i>
書誌事項
- タイトル別名
-
- Biological Activity of Brain-derived Neurotrophic Factor with Mismatched Disulfide Linkages Produced by Escherichia coli.
この論文をさがす
説明
E. coli cells harboring an expression plasmid with a brain-derived neurotrophic factor (BDNF) gene obtained from rat BDNF cDNA were sonicated and centrifuged to obtain a precipitate containing BDNF. Ten different proteins of BDNF were purified from the precipitate and the three disulfide linkages of six proteins were identified. Those disulfide structures were different from that of authentic BDNF and the biological activities of BDNF with mismatched disulfide linkages (EC50 of 2 to 15ng/ml) were much lower than that of authentic BDNF (EC50 of 30 pg/ml). The BDNF with mismatched disulfide linkages inhibited the biological activity of authentic BDNF.
収録刊行物
-
- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
-
Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 60 (6), 971-974, 1996
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681450460416
-
- NII論文ID
- 110002678171
-
- NII書誌ID
- AA10824164
-
- COI
- 1:CAS:528:DyaK28XjvFGhsro%3D
-
- ISSN
- 13476947
- 09168451
-
- PubMed
- 8695913
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- JaLC
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可