Identification of a Structural Gene Encoding a Metallothionein-like Domain that Includes a Putative Regulator Protein for Streptomyces Protease Gene Expression.

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  • TAGUCHI Seiichi
    Department of Biological Science and Technology, Science University of Tokyo
  • OGAWA Takahiro
    Department of Biological Science and Technology, Science University of Tokyo
  • ENDO Takeshi
    Department of Biological Science and Technology, Science University of Tokyo
  • MOMOSE Haruo
    Department of Biological Science and Technology, Science University of Tokyo

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  • Identification of a Structural Gene Encoding a Metallothionein-like Domain that Includes a Putative Regulator Protein for<i>Streptomyces</i>Protease Gene Expression

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An open reading frame (termed ORF-PR) encoding a metallothionein-like domain-including protein was found upstream of a previously identified Streptomyces chymotrypsin-type protease gene (sam-P20). Promoter and terminator activities of ORF-PR were detected using the promoterless Streptomyces tyrosinase gene as a reporter gene and expression of ORF-PR was supposed to occur before that of sam-P20 gene. Frameshift mutation anaysis showed that the ORF-PR product might act as a repressive regulator of the sam-P20 gene.<br>

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