DNA Synthesis and Fragmentation in Bacteroids during Astragalus sinicus Root Nodule Development.
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- KOBAYASHI Hajime
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- SUNAKO Michihiro
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- HAYASHI Makoto
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- MUROOKA Yoshikatsu
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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Abstract
Histo- and cytochemical techniques were used to study the DNA replication and fragmentation patterns in bacteroids formed by Mesorhizobium huakuii subsp. rengei in nodules of Astragalus sinicus. DNA replication was detected by the incorporation of 5-bromo deoxy-uridine. Signals denoting DNA synthesis were observed in plant nuclei within the nodule meristem and in bacteroids near the meristem. The TUNEL (TdT-mediated dUTP nick-end labeling) assay was used to measure DNA fragmentation. In nutrient-depleted 1-mpi (month(s) post inoculation) nodule sections, some bacteroids were in vacuoles, and DNA fragmentation signals were observed only in such bacteroids. In contrast, 1-mpi nodule sections without nutrient depletion showed neither bacteroid localization in vacuoles nor DNA fragmentation signals. The bacteroid translocation into vacuoles upon nutrient starvation might results from autophagy of the plant. In 2-mpi nodule sections, bacteroids with DNA fragmentation signals appeared within the cytoplasm of some nodule cells in the senescence zone.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 65 (3), 510-515, 2001
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681450518016
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- NII Article ID
- 110002680296
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3MXisFKqtLc%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 5736485
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- PubMed
- 11330661
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed