Cloning, Expression, and Characterization of a Family 52β-Xylosidase Gene (xysB) of a Multiple-xylanase-producing Bacterium, Aeromonas caviae ME-1
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- SUZUKI Tohru
- Molecular Genetics Research Center
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- KITAGAWA Emiko
- Department of Biotechnology, Faculty of Agriculture, Gifu University
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- SAKAKIBARA Fukumitsu
- Department of Biotechnology, Faculty of Agriculture, Gifu University
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- IBATA Keiji
- Department of Biotechnology, Faculty of Agriculture, Gifu University
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- USUI Kengo
- Department of Biotechnology, Faculty of Agriculture, Gifu University
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- KAWAI Keiichi
- Department of Biotechnology, Faculty of Agriculture, Gifu University
書誌事項
- タイトル別名
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- Cloning, Expression, and Characterization of a Family 52.BETA.-Xylosidase Gene(xysB) of a Multiple-xylanase-producing Bacterium, Aeromonas caviae ME-1.
- Cloning Expression and Characterization of a Family 52 ベータ Xylosidase Gene xysB of a Multiple xylanase producing Bacterium Aeromonas caviae ME 1
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説明
A λ phage genomic library of Aeromonas caviae ME-1, a multiple-xylanase-producing bacterium, was screened for xylan degradation activities. We isolated one clone, B65, which had weak xylanase activity, by the DNS method, but gave no visible bands on zymogram assay using SDS-xylan-PAGE. Based on TLC analyses of enzymatic products and some glycosidase assays using p-nitrophenyl substrates, we established that pB65 encodes a β-xylosidase gene. In the nucleotide sequence analysis, we found a 2190-bp open reading frame (ORF) named xysB. XysB protein is similar to some β-xylosidases, which are categorized in the glycosyl hydrolase family 52. Another ORF (xyg), that showed similarity to the family 67 α-glucuronidase, was also found downstream of the xysB gene. The xysB ORF and its promoter region were cloned into the pT7-Blue vector and the transformant cells had β-xylosidase activity. The relative molecular mass were estimated to be 75 kDa by SDS-PAGE and 159 kDa by gel filtration. These data showed that XysB has a dimeric structure of 80, 697 Da subunits. This enzyme showed optimal activity at 50°C and pH 6.0. It was stable below 40°C and pH 5-8. The Km and Vmax were calculated to be 0.34 mM and 33 nmol·min-1·μg-1, respectively. This enzyme also showed transglycosylation activity against X3 and produced X4 and X5.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 65 (3), 487-494, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681450528256
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- NII論文ID
- 110002680293
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3MXisFKqtLg%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5736282
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- PubMed
- 11330658
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可