Human Lysozyme Secretion Increased by Alpha-factor Pro-sequence in<i>Pichia pastoris</i>
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- OKA Chitoshi
- Chiba Prefectural Industrial Research Institute
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- TANAKA Masao
- Chiba Prefectural Industrial Research Institute
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- MURAKI Michiro
- National Institute of Bioscience and Human-Technology
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- HARATA Kazuaki
- National Institute of Bioscience and Human-Technology
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- SUZUKI Katsunori
- Department of Biological Science, Faculty of Science, Hiroshima University
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- JIGAMI Yoshifumi
- National Institute of Bioscience and Human-Technology
書誌事項
- タイトル別名
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- Human Lysozyme Secretion Increased by Alpha-factor Pro-sequence in Pichia pastoris.
- Human lysozyme secretion increased by α-factor pro-sequence in Pichia pastoris
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説明
To get high level secretion of human lysozyme in Pichia pastoris, the following three signal sequences and one prepro sequence were evaluated: chicken lysozyme signal peptide, leucine-rich artificial signal peptide, Saccharomyces invertase signal peptide, and Saccharomyces prepro sequence of alpha factor (MF-α Prepro).<br> Transformants harboring a lysozyme gene with MF-α Prepro secreted 20-fold more lysozyme than those harboring the lysozyme gene with any one of the other three signal sequences. Three mutant leader sequences derived from MF-α Prepro were constructed to discover the function of the pro region. The secretion was dramatically decreased by eliminating the pro region of MF-α Prepro. In contrast, MF-α Prepro with the EAEAEA sequence directed the secretion of an equivalent level of lysozyme having the extra amino acids (EAEAEA) in its N-terminus.<br> For the effective secretion of native human lysozyme, MF-α Prepro without any spacer sequences was most suitable. The secreted protein by MF-α Prepro construct was identical with the authentic human lysozyme, judging from N-terminal amino acid sequencing and molecular mass spectrometric and crystallographic analysis.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 63 (11), 1977-1983, 1999
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681450551040
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- NII論文ID
- 110002679398
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1MXnvVCktLw%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4929956
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- PubMed
- 10635562
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- NDLサーチ
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- PubMed
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- 使用不可