Anti-microbial Action against Verotoxigenic Escherichia coli O157:H7 of Nitric Oxide Derived from Sodium Nitrite
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- MORITA Hidetoshi
- School of Veterinary Medicine, Azabu University
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- YOSHIKAWA Hiroshi
- School of Veterinary Medicine, Azabu University
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- SUZUKI Takehito
- School of Veterinary Medicine, Azabu University
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- HISAMATSU Shin
- School of Environmental Health Science, Azabu University
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- KATO Yukio
- School of Veterinary Medicine, Azabu University
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- SAKATA Ryoichi
- School of Veterinary Medicine, Azabu University
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- NAGATA Yukiharu
- School of Veterinary Medicine, Azabu University
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- YOSHIMURA Tetsuhiko
- Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry
Bibliographic Information
- Other Title
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- Anti-microbial Action against Verotoxigenic Escherichia coli O157:H7 of Nitric Oxide Derived from Sodium Nitrile
- Anti-microbial Action against Verotoxigenic<i>Escherichia coli</i>O157:H7 of Nitric Oxide Derived from Sodium Nitrite
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Abstract
The levels of verotoxin-1 and verotoxin-2 released by verotoxigenic Escherichia coli O157:H7 treated in vitro with sodium nitrite, sodium chloride and several antibiotics were evaluated. Of the three strains of E. coli O157:H7 used in this study, two strains produced both verotoxin-1 and verotoxin-2, and one strain produced only verotoxin-2. Treatment of E. coli O157:H7 with sodium nitrite (6,000 mg/l, minimum inhibitory concentration) did not increase the levels of verotoxin-1 and verotoxin-2 compared with a treatment by sodium chloride or antibiotics. When the electron paramagnetic resonance spectrum of sodium nitrite-treated bacterial cells was examined at 77 K to clarify the mechanism for the anti-bacterial activity of nitric oxide derived from sodium nitrite, electron paramagnetic resonance signals with g-values of 2.035 and 2.010 were observed. These were identified as being derived from iron–nitric oxide complexes. It appears that the dinitrosyl iron complexes in the E. coli O157:H7 cells were generated from the reaction of iron-sulfur proteins (enzymes) with nitric oxide formed by the reduction of sodium nitrite. The amount of ATP was decreased by the presence of sodium nitrite in the cell suspension. These findings indicate that nitric oxide derived from sodium nitrite penetrated the cells and inactivated enzymes related to the respiratory chain.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (5), 1027-1034, 2004
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681450605696
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- NII Article ID
- 10013144505
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 6957387
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- PubMed
- 15170105
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed