Gene Encoding a Dextransucrase-like Protein in Leuconostoc mesenteroides NRRL B-512F.
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- FUNANE Kazumi
- Carbohydrate Science Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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- MIZUNO Kouichi
- Carbohydrate Science Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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- TAKAHARA Hidenari
- Department of Resource Biology, Faculty of Agriculture, Ibaraki University
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- KOBAYASHI Mikihiko
- Akita Research Institute of Food and Brewing
Bibliographic Information
- Other Title
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- Gene Encoding a Dextransucrase-like Protein Leuconostoc mesenteroides NRRL B-512F
- Gene Encoding a Dextransucrase-like Protein in<i>Leuconostoc mesenteroides</i>NRRL B-512F
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Abstract
A gene, dsrT, encoding a dextransucrase-like protein was isolated from the genomic DNA libraries of Leuconostoc mesenteroides NRRL B-512F dextransucrase-like gene. The gene was similar to the intact open reading frames of the dextransucrase gene dsrS of L. mesenteroides NRRL B-512F, dextransucrase genes of strain NRRL B-1299 and streptococcal glucosyltransferase genes, but was truncated after the catalytic domain, apparently by the deletion of five nucleotides. dsrT mRNA was produced in this strain L. mesenteroides when cells were grown in a sucrose medum, but at a level of 20% of that of dsrS mRNA. The molecular weight of the dsrT gene product was 150,000 by SDS-PAGE. The product did not synthesize dextran, but had weak sucrose cleaving activity. The insertion of five nucleotides at the putative deletion point in dsrT resulted in an enzyme with a molecular weight of 210,000 and with dextransucrase activity.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (1), 29-38, 2000
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681450747648
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- NII Article ID
- 110002679743
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXhtVyitb0%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- NDL BIB ID
- 5143614
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- PubMed
- 10705445
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed