Safety Assessment of Genetically Engineered Food: Detection and Monitoring of Glyphosate-Tolerant Soybeans.
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- SHIRAI Nobuaki
- Industrial Research Center of Shiga Prefecture
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- MOMMA Keiko
- Research Institute for Food Science, Kyoto University
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- OZAWA Sachiko
- Research Institute for Food Science, Kyoto University
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- HASHIMOTO Wataru
- Research Institute for Food Science, Kyoto University
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- KITO Makoto
- Research Institute for Food Science, Kyoto University
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- UTSUMI Shigeru
- Research Institute for Food Science, Kyoto University
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- MURATA Kousaku
- Research Institute for Food Science, Kyoto University
書誌事項
- タイトル別名
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- Safety Assessment of Genetically Engine
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抄録
A detection technique for the genetically engineered food, glyphosate-tolerant soybean (GTS), was designed. Commercial soybeans imported from North America were cultured in pots and genomic DNA was isolated from their leaves. To detect the genes, promoter and terminator, involved in the expression of glyphosate tolerance, PCR was done using the genomic DNA and chemically synthesized primers specific to the genes. DNAs with predicted sizes were amplified and confirmed by DNA sequencing to be the genes responsible for the expression of glyphosate tolerance. Glyphosate-tolerant soybeans were found to form approximately 1.1% of the commercial soybeans, when commercially available soybeans were cultivated and number of soybeans resistant to glyphosate was found. This level is somewhat lower than an estimated value announced officially on the basis of the cultivation area of the glyphosate-tolerant soybeans.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 62 (7), 1461-1464, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681450910208
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- NII論文ID
- 110002691981
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXltFGhurk%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4538405
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- PubMed
- 9720233
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可